Cation channel homologues in pathogenic fungi.

Protein accession numbers are shown, except in the case of H. capsulatum for which transcript identifiers are shown (NCBI and Broad Institute of Harvard and MIT, see Methods). MCU denotes the human mitochondrial Ca2+ uniporter (NP_612366). Genes encoding homologues of MCU are also found in the genomes of: the Ascomycota Aspergillus spp., Fusarium spp., Verticillium spp., Chaetomium globosum, Neurospora crassa, Magnaporthe grisea, Botrytis cinerea, Sclerotinia sclerotiorum, Stagonospora nodorum, and Pyrenophora tritici-repentis; the Basidiomycota Cryptococcus spp., C. cinerea and Ustilago maydis; and the Chytridiomycota A. macrogynus and Spizellomyces punctatus. In contrast, genes encoding MCU homologues appear to be absent from the genomes of other fungi such as E. cuniculi, E. intestinalis, E. bineusi, Saccharomyces spp., Schizosaccharomyces spp., Microsporum spp., and other species of Trichophyton. Homologues of MICU1 (NP_006068), the Ca2+-sensing modulatory subunit of MCU, are also encoded by some fungal genomes, including (protein accession number or transcript identifier shown in parentheses): T. rubrum (XP_003233268), A. clavatus (XP_001273355), A. flavus (EED56817), A. fumigatus (XP_748987), C. immitis (XP_001245264), C. posadasii (XP_003071580), P. brasiliensis (XP_002792408), H. capsulatum (HCEG_05324.2), B. dermatitidis (EGE79123.1), C. gattii (XP_003192784) and C. neoformans (XP_569565), but appear to be absent from the other genomes examined. Homologues of the Cch1 auxiliary subunit Mid1 (NP_014108) in S. cerevisiae are also found in the following fungi: T. rubrum (XP_003235133.1), A. clavatus (XP_001273916), A. flavus (EED46777), A. fumigatus (XP_754048), C. immitis (XP_001242343), C. posadasii (XP_003069581), P. brasiliensis (XP_002790830), C. albicans (XP_710963), C. glabrata (XP_449502), C. tropicalis (XP_002551139), H. capsulatum (HCEG_04307.2), B. dermatitidis (BDDG_05843.1), C. gattii (XP_003192201) and C. neoformans (XP_569171). The number of predicted transmembrane domains in each protein is indicated in parentheses. For homologues of K+ channel subunits, the predicted family of K+ channel (K2P or Kv) is also indicated in parentheses. In addition to those shown, Kv channel subunit homologues were also identified in: the Basidiomycota Coprinopsis cinerea (XP_002910836), Laccaria bicolour (XP_001881176), Serpula lacrymans (EGN93868) and Postia placenta (EED81504); the Chytridiomycete Allomyces macrogynus (AMAG_10122.1, AMAG_16737.1, AMAG_16515.1, AMG_06554.1 and AMAG_15091.1); and the Zygomycete Rhizopus oryzae (RO3G_09031.3). The presence (+) or apparent absence (−) of homologues of MICU1 is indicated for each fungal genome, shown in parentheses after the MCU homologue annotation. NF denotes no homologues found.