Data for: OsDSSR1, a novel small peptide, enhances drought tolerance in transgenic rice

RNA-seq analysis was carried out using transgenic lines OX37. Fourteen-day-old rice seedlings were treated with or without 20% solutions of PEG6000 for 6h. Total RNA was isolated with Trizol reagent (Invitrogen, USA) from seedlings of transgenic and 93-11 plants grown under normal or PEG conditions. Material from 10 plants of each genotype was pooled for RNA extraction. The concentration and quality of each RNA sample were determined using a NanoDrop 2000TM micro-volume spectrophotometer (Thermo Scientific, Waltham, MA, USA) and gel electrophoresis. The rice RNA samples were sent to BGI (The Beijing Genomics Institute at Shenzhen, China) for sequencing.本研究以转基因株系OX37为材料开展RNA测序（RNA-seq）分析。选取14日龄水稻幼苗，分别以20% PEG6000溶液处理6小时，同时设置未施加PEG6000的空白对照组。分别从正常培养及PEG胁迫处理的转基因株系与93-11水稻幼苗中，采用Trizol试剂（美国Invitrogen公司）提取总RNA；每份基因型取10株水稻幼苗的材料混合后进行RNA提取。利用NanoDrop 2000TM超微量分光光度计（美国马萨诸塞州沃尔瑟姆市赛默飞世尔科技公司）及琼脂糖凝胶电泳，检测每份RNA样品的浓度与质量。将提取的水稻总RNA样品送至BGI（中国深圳北京基因组研究所）进行测序。