Single cell analysis of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin Epcam- cells and CD45-Epcam- stromal cells from lamina propria at the age of 11 wks.
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE173565
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Comparison of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin Epcam- cells and CD45-Epcam- stromal cells from lamina propria at the age of 11 wks. Two male mice per group was used in the experiment. We analyzed two samples, aiming with both samples to 8,000-10,000 cells. EDTA treatment 20min +37°C. Liberase TL treatment 60min +37°C. FACS: DAPI-Ter119-Epcam-CD45- intestinal stromal cells
创建时间：
2021-12-11
