Microalgal 18S rRNA gene community dynamics in agricultural ponds fertilized to stimulate blooms for biofuel generation

Man-made freshwater ponds were filled with natural stream water in Frederick, MD, USA and fertilized with chicken manure to stimulate blooms of microalgae. Microalgae biomass would subsequently be harvested and converted into biofuel. The 18S rRNA gene community was monitored in the water overtime to track which microalgal communities were most responsive to the eutrophication of the water. In a parallel study, the bacterial 16S rRNA gene community was also studied.