Gas lean obob GRf/fGRmKO

Muscle glucocorticoid receptor (GR) signaling regulates gene expressions in skeletal muscle. To reveal the roles of muscle GR in systemic metabolism in obesity, ob/ob background muscle-specific GR knockout (ob/ob GRmKO) mice were generated. GR Flox+/+ Cre-/- Lep+/+ (lean GRf/f), GR Flox+/+ Cre+/- Lep+/+ (lean GRmKO), GR Flox+/+ Cre-/- Lepob/ob (ob/ob GRf/f), and GR Flox+/+ Cre+/- Lepob/ob (ob/ob GRmKO) were compared. Gastrocnemius muscle from 7-week-old male mice was excised and analyzed. We used pooled RNA samples (n = 3).