Neochromosomes: Creating transcriptional units with yeast Golden Gate assembly

This resource is a series of protocols using the yeast Golden Gate (yGG) method as developed by Neta Agmon and Leslie Mitchell in Jef Boeke’s lab to assemble a functional yeast gene with protein-coding and regulatory sequences.

本资源为一系列采用酵母Golden Gate（yGG）方法（由Neta Agmon和Leslie Mitchell在Jef Boeke实验室开发）构建功能酵母基因的协议，该基因包含编码序列和调控序列。