Wolbachia impacts microbiome diversity and fitness-associated traits for Drosophila melanogaster in a seasonally fluctuating environment

The microbiome contributes to many different host traits, but its role in host adaptation remains enigmatic. The fitness benefits of the microbiome often depend on ecological conditions, but theory suggests that fluctuations in both the microbiome and environment modulate these fitness benefits. Moreover, vertically transmitted bacteria might constrain the ability of both the microbiome and host to respond to changing environments. Drosophila melanogaster provides an excellent system to investigate the impacts of interactions between the microbiome and the environment. To address this question, we created field mesocosms of D. melanogaster undergoing seasonal environmental change with and without the vertically transmitted bacteria, Wolbachia pipientis. Sampling temporal patterns in the microbiome revealed that Wolbachia constrained microbial diversity. Furthermore, Wolbachia and a dominant member of the microbiome, Commensalibacter, were associated with differences in two higher-order ..., , , # data for Wolbachia impacts microbiome diversity and fitness-associated traits for Drosophila melanogaster in a seasonally fluctuating environment

[https://doi.org/10.5061/dryad.547d7wmg3](https://doi.org/10.5061/dryad.547d7wmg3)

Data was generated from a study of Drosophila undergoing seasonal adaptation in NJ during summer-fall 2019.

## Description of the data and file structure

**Data for temperature data loggers** are in files called \"`dl_N.csv`\", with N = 1-8 to note the different cages. Each column for the data loggers is as follows: S/N = arbitrary sorting, Time = time the measurement was collected, Temperature = temperature in Celsius, Humidity%RH = %relative humidity. 

**The following files are for microbiome data (generated using QIIME2 and exported to analyses/visualization in R)**:
`field19_total_meta.tsv` is the metadata that relates sequencing library to collected metadata. The columns are as follows: flyid = individual fly, BarcodeSequence = is for demultiplexing, f...