Tead1 regulation of ß-cells II

Purpose: The goal of this study is to determine the regulatory role of Tead1 in ß-cells by analyzing the Tead1 cistrome and open chromatin in ß-cells Methods: Isolated islets from WT C57bl6 mice of 12 weeks of age were flash frozen. For Chip-seq they were fixed with formaldehyde and then after sonication, IP was performed with Anti-Tead1 antibody or the IgG isotype control. For ATAC-seq 100,000 of the frozen nuclei were tagmented. After DNA extraction, library construction, sequencing was performed using Illumina Hi seq2500. Conclusions: Our study represents the first detailed analysis of the Tead1 cistrome in beta cells. Overall design: islated islets from 20 mice were pooled into two separate pools of 10 each and processed for ChIP-seq and concurrent ATAC-seq