scRNAseq comparison of iPSC-derived retinal organoids from Control and LCA7 patients. scRNAseq comparison of iPSC-derived retinal organoids from Control and LCA7 patients

To comprehensively capture changes in retinal transcriptome for the LCA7 organoids compared to control, we performed single cell RNA-sequencing (scRNAseq) using the 10X Genomics platform. Retinal organoids at D150 of differentiation were dissociated for scRNAseq analysis. scRNAseq data revealed significant dysregulation of specific photoreceptor genes between control and LCA7 organoids, as well as mutation-specific differences in various genes, including CRX, RCVRN, ARR3, and AIPL1. Overall design: Comparison of iPSC-derived retinal organoids from 1 Control and 2 LCA7 patients with two different mutations: K99Q and T155ins4