Human liver organoids; a patient-derived primary model for HBV Infection, Replication and Related Hepatocellular Carcinoma

Here we interrogate the potential of human liver organoids as a platform for modeling HBV infection and related tumorigenesis. We show that organoids derived from HBV-infected patients display an aberrant early cancer gene signature, which clusters with the HCC cohort on the TCGA LIHC dataset and away from healthy liver tissue. Total RNA obtained from HBV infected patient-derived or healthy donor liver organoids