Quantitative eDNA metabarcoding survey at the Hebisawagawa front reservoir

The survey sites were located in the Hebisawagawa front reservoir at the Miharu Reservoir in Fukushima Prefecture, Japan. The total area of the Hebisawagawa front reservoir was about 44,000 m2. This front reservoir is connected to the main reservoir by a channel with a width of 5 m and a depth of 5 m. It is isolated during the summer season (June 11th to October 10th) when the water level is low. Water samples were collected at three sites once a week from March 26 to August 13, 2019, and March 3 to August 25, 2020. Quantitative metabarcoding approach was used to simultaneously quantify the eDNA concentration of multiple fish species.