RNA-Seq expression profiling of mouse DEN-initiated hepatocytes with or without Tgfbr2 inactivation

Initiated hepatocytes (IHCs), isolated from DEN exposed Tgfbr2 flox/flox mouse, were infected with GFP or Cre-expressed adenovirus, followed by 1ng/ml TGFβ × 48 hours incubation. Then total RNAs were isolated and processed to routine sequencing on Illumina platform. RNA-Seq libraries were prepared from total RNA using polyA enrichment. Examination of transcriptome differences between initiated hepatocytes with or without Tgfbr2 inactivation.