Single RNA-seq using choroidal tissue of murine choroidal neovascularization model

Mice were divided randomly into control and CNV group. CNV model was induced by laser photocoagulation. Single cell suspensions were prepared 7 days after, using choroidal tissues from control mice (normal group) and photocoagulated eye (CNV group) or opposite eye (Ctrl group) from CNV mice. Gene expression library construction and sequencing were performed using the Chromium (10X Genomics) instrument and Illumina Novaseq 6000