The circadian clock protein Cryptochrome 1 is a direct target and feedback regulator of the Hippo pathway

To examine the impact of Cry1 downregulation on gene expression, empty vector (Sh Ctrl) and Sh-RNA targeting Cry1 (Sh Cry1) were used to produce lentiviral vector. Cell were then infected with control and sh-RNA lentivirus and then selected with puromycin. Upon validation of the knockdown with real time PCR. RNA were prepared from cells stably expressing empty vector or sh-RNA against Cry1 and used gene expression profiling by RNA seq. RNA of two biological replicate were prepared from each genotype. Overall design: To examine the impact of Cry1 downregulation on gene expression empty vector (Sh Ctrl) and Sh-RNA targeting Cry1 (Sh Cry1) were used to produce lentiviral vector.