Enzymes immobilized in Langmuir-Blodgett films: Why determining the surface properties in Langmuir monolayer is important?

ABSTRACT In this review we discuss about the immobilization of enzymes in Langmuir-Blodgett films in order to determine the catalytic properties of these biomacromolecules when adsorbed on solid supports. Usually, the conformation of enzymes depends on the environmental conditions imposed to them, including the chemical composition of the matrix, and the morphology and thickness of the film. In this review, we show an outline of manuscripts that report the immobilization of enzymes as LB films since the 1980’s, and also some examples of how the surface properties of the floating monolayer prepared previously to the transfer to the solid support are important to determine the efficiency of the resulting device.摘要 本综述探讨了酶在朗缪尔-布吉特（Langmuir-Blodgett）膜中的固定化技术，旨在研究这类生物大分子吸附于固体载体时的催化性能。通常，酶的构象取决于其所受的环境条件，包括基质的化学组成、薄膜的形貌与厚度。本综述梳理了自20世纪80年代以来，以LB膜形式固定化酶的相关文献概况，并通过若干实例阐释了在转移至固体载体前制备的浮动单分子层的表面性质对所得器件催化效率的关键影响。