Chronic exposure water chemistry data

To fully assess the impacts of Deepwater Horizon (DWH) oil on the vertebrate stress response and the trajectory for recovery, our research integrated molecular, cellular, gland/organ and whole animal physiological and behavioral studies using a benthic marine teleost, the Gulf toadfish, a resident of the Gulf of Mexico. These studies evaluated multiple pathways (e.g., pituitary fatigue, reduced receptor sensitivity, disruption or overstimulation of cortisol biosynthesis) and the consequence of these changes at the whole animal level (e.g., changes in carbohydrate metabolism, immune capacity and responses to natural stresses such as predation and social interaction). In this particular experiment, the Gulf toadfish was exposed to a concentration of polycyclic aromatic hydrocarbon (PAH) for 28 days followed by a period of 28 days recovery. The temperature, salinity and dissolved oxygen concentrations in the water column were measured. Fluorescence was also measured. These experiments were completed at Rosenstiel School of Marine and Atmospheric Science (RSMAS), University of Miami, Marine Technology & Life Sciences Seawater Complex (MTLSS) Building, room 211 between January 2019 to December 2019.为全面评估深水地平线（Deepwater Horizon, DWH）原油对脊椎动物应激反应的影响及恢复轨迹，本研究以墨西哥湾本土底栖海水硬骨鱼类——海湾豹蟾鱼（Gulf toadfish）为实验对象，整合了分子、细胞、腺体/器官及整体动物层面的生理学与行为学研究。本研究评估了多条应激通路（如垂体疲劳、受体敏感性降低、皮质醇生物合成紊乱或过度刺激），并探究了上述变化在整体动物层面产生的后果，包括碳水化合物代谢、免疫能力的改变，以及对捕食、社会互动等自然应激源的反应变化。本次特定实验中，海湾豹蟾鱼被暴露于多环芳烃（polycyclic aromatic hydrocarbon, PAH）环境中28天，随后开展为期28天的恢复阶段。实验期间对水体的温度、盐度及溶解氧浓度进行了测定，同时也测定了荧光值。本系列实验于2019年1月至2019年12月间，在迈阿密大学罗森斯蒂尔海洋与大气科学学院（Rosenstiel School of Marine and Atmospheric Science, RSMAS）海洋技术与生命科学海水综合实验楼（Marine Technology & Life Sciences Seawater Complex, MTLSS）211室完成。