Structure of D169A/E171A double mutant of chitinase Chit42 from Trichoderma harzianum complexed with chitintetraose obtained by soaking.

Structure of D169A/E171A double mutant of chitinase Chit42 from Trichoderma harzianum complexed with chitintetraose obtained by soaking. Descriptor: 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ACETATE ION, ... Authors: Jimenez-Ortega, E, Sanz-Aparicio, J. Deposit date: 2020-10-01 Release date: 2021-10-20 Last modified: 2024-01-31 Method: X-RAY DIFFRACTION (2.32 Å) Cite: Structural inspection and protein motions modelling of a fungal glycoside hydrolase family 18 chitinase by crystallography depicts a dynamic enzymatic mechanism Comput Struct Biotechnol J, 19, 2021

本数据集为通过浸泡法获得的哈茨木霉（Trichoderma harzianum）来源几丁质酶（chitinase）Chit42的D169A/E171A双突变体与几丁四糖（chitintetraose）复合物的晶体结构。配体描述：2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖-(1→4)-2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖、2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖-(1→4)-2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖-(1→4)-2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖-(1→4)-2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖、乙酸根离子（ACETATE ION）等。作者：Jimenez-Ortega, E、Sanz-Aparicio, J。提交日期：2020-10-01；发布日期：2021-10-20；最后修改日期：2024-01-31。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率2.32埃（Å）。引用文献：通过晶体学对真菌糖苷水解酶家族18（glycoside hydrolase family 18）几丁质酶开展结构解析与蛋白质运动建模，揭示动态酶促机制，发表于《计算与结构生物技术期刊》（Comput Struct Biotechnol J），2021年，第19卷。