Chicken single cell seq gene expression matrix

WGAhigh CAM cells were sorted by FACS into PCR tubes. Single-cell RNA-seq experiments were performed according to the Smart-seq2 protocol with 20 cDNA pre-amplification cycles. Samples were sequenced by Illumina Hiseq 4000 with 150-bp paired-end reads. The transcriptome was quantified by Salmon with the chicken genome reference GRCg6a. Data from cells with more than 1500 genes detected were considered as high quality and were used for subsequent analysis. Further data analysis and visualization used Seurat.