Single-cell data of "Growth rate controls the sensitivity of gene regulatory circuits"

NIAID Data Ecosystem2026-05-02 收录

下载链接：

https://zenodo.org/record/7429483

下载链接

链接失效反馈

官方服务：

资源简介：

Single-cell data of microfluidic experiments reported in the article Growth rate controls the sensitivity of gene regulatory circuits. The corresponding image dataset, together with raw export files from the image analysis software MoMA, is available at https://doi.org/10.5281/zenodo.6684959. Two series of experiments of have been conducted, where bacterial growth and lac operon expression are monitored using phase contrast and epifluorescence microscopy (measuring a translational LacZ-GFP fusion): "Transient growth arrest": Escherichia coli cells are exposed to M9 minimal medium with glycerol, and then to media containing TMG inducer either with glycerol or with lactulose (data from 4 independent experiments). "Concentration-dependent glucose/lactose preference": Escherichia coli cells pre-grown in M9 minimal medium with 0.2% glucose are grown in mother machine microfluidic chips in one of the following conditions (data from 6 independent experiments with up to 8 conditions per experiments): lactose 0.02% with variable glucose concentration (2µM to 128µM) variable glucose concentration (2µM to 128µM) variable glucose concentration (2µM to 128µM) with 200µM IPTG. Each subdirectory corresponds to a given date and conditions as described below (also available in Julou_2022_GCS_series.csv). A list of data files is provided in Julou_2022_GCS_GL_Preproc_fileList.txt. subdirectory,condition 20180712,switch_glycerol_TMG20 20181008,switch_lactulose_TMG20 20181009,switch_lactulose_TMG20 20181024,switch_lactulose 20210122_S0,glu2uM 20210122_S1,lac002 20210122_S2,lac002_glu2uM 20210122_S3,lac002_glu4uM 20210122_S4,lac002_glu8uM 20210122_S5,lac002_glu16uM 20210122_S6,lac002_glu32uM 20210122_S7,lac002_glu002 20210305_S0,glu2uM 20210305_S1,lac002 20210305_S2,lac002_glu2uM 20210305_S3,lac002_glu4uM 20210305_S4,lac002_glu8uM 20210305_S5,lac002_glu16uM 20210305_S6,lac002_glu32uM 20210305_S7,lac002_glu002 20210504_S0,lac002_glu16uM 20210504_S1,lac002_glu32uM 20210504_S2,lac002_glu64uM 20210504_S3,lac002_glu128uM 20210504_S4,glu2uM+IPTG 20210504_S5,glu8uM+IPTG 20210504_S6,glu32uM+IPTG 20210506_S2,lac002_glu64uM 20210506_S3,lac002_glu128uM 20210506_S4,glu2uM+IPTG 20210506_S5,glu8uM+IPTG 20210506_S6,glu32uM+IPTG 20210506_S7,glu128uM+IPTG 20210513_S0,glu2uM 20210513_S1,glu4uM 20210513_S2,glu8uM 20210513_S3,glu16uM 20210513_S4,glu32uM 20210513_S5,glu64uM 20210513_S6,glu2uM+IPTG 20210513_S7,glu128uM+IPTG 20210708_S0,glu8uM 20210708_S1,glu32uM 20210708_S2,glu128uM 20210708_S4,glu002 20210708_S5,M9zero 20210708_S6,glu02 20210708_S7,lac002_glu128uM

创建时间：

2025-02-07