18S rRNA gene amplicon sequencing of soil eukaryotes under agricultural management regimes

Here we present the data from a metabarcoding study of soil eukaryotic communities using primers targeting the V9 region of the 18S rRNA gene (1380F/1510R). Soil samples were collected in spring 2021 from a randomized block experiment conducted at Foulumgaard, Tjele, Denmark. The experimental design included two tillage methods (plowing and direct seeding), three fertilizer types (granular mineral fertilizer, urea-ammonium nitrate, and pig slurry), and three nitrification inhibitors (N-Lock, Vizura, and Piadin). In total, 60 bulk soil samples were sequenced to characterize community composition based on environmental DNA.