Real-time RT-PCR analysis of mRNA decay: half-life of Beta-actin mRNA in human leukemia CCRF-CEM and Nalm-6 cell lines

Background We describe an alternative method to determine mRNA half-life (t1/2) based on the Real-Time RT-PCR procedure. This approach was evaluated by using the...研究背景：我们提出了一种基于实时逆转录聚合酶链反应（Real-Time RT-PCR）流程的替代方法，用于测定信使核糖核酸（mRNA）的半衰期（t1/2）。本研究通过使用……对该方法进行了评估。