Microbe abundance and biomass in the euphotic zone of the Aegean Sea at Station MARCH-1997-GN36199704601MSB01 in spring 1997@en
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10-30 ml subsamples were concentrated on 25 mm black polycarbonate filters (0.6 µm for NF, 0.2 µm for B), stained with DAPI for 10 min and filtered. NF were counted using UV and blue excitation and enumerated. Heterotrophic B were counted using UV excitation. NF were classified in size categories and biovolume was calculated. […]
取10~30毫升子样本,在25毫米黑色聚碳酸酯滤膜(NF组采用0.6 μm孔径滤膜,B组采用0.2 μm孔径滤膜)上完成浓缩,随后以4',6-二脒基-2-苯基吲哚(DAPI)染色10分钟后进行过滤。采用紫外与蓝光激发对NF进行计数并枚举。异养细菌(Heterotrophic B)采用紫外激发完成计数。对NF按尺寸类别进行分类,并计算其生物体积。[…]
创建时间:
2026-04-24



