Germination time and maximum seed viability in CAX mutants.

Seeds from Col-0 (wild type) and cax knockout mutant plants (approximately 70 of each line) were sterilized and sown on 1% agar before stratification for 2 d at 4°C in the dark. Plates were incubated (22°C, 16 h light/8 h dark) for 9 h then seeds were observed every 2 h for 12 h, then every 12 h until 10 d after which any seeds not germinated were considered non-viable. All values are the mean of three replicate experiments.