SMRT-seq for m6A methylation analysis dataset for Pseudomonas aeruginosa PAO1 37°C vs. 43°C

The data in this item includes Single-molecule real-time sequencing (SMRT-seq) data generated using PacBio Sequel I for high-resolution analysis of m6A methylation from post-43°C grown bacteria and during the recovery period.Sample preparation: Overall, 15 samples included 3 replicates of Pseudomonas aeruginosa after exposure to 37 °C (control) or 43 °C at 4 time points (T = 0 generations, T = 5 generations, T = 22 generations, and T = 54 generations). Samples from the T=0 groups are simply labeled “37” or “43”.Analysis: Pac Bio subreads were mapped to the reference genome Pseudomonas aeruginosa PAO1 GCA000006765, ASM676v1, for each sample separately, using the pbmm2 tool from the smrttools 10 toolkit (Pac Bio). m6A modifications were then inferred with the ipdSummary tools. The control model was used in two separate runs. In the first run samples from the group 43 were used as reference. In the second run, samples from the group 37 were used as reference. Sites with ipd-ratio with p-value