Real time PCR analysis during thermal manipulation in mule duck

This study proposes to describe impacts of an increase of temperature during embryogenesis by following hepatic metabolism at the level of mRNA in mule duck embryos Two kinetic studies were designed to determine the level of expression of selected genes involved in liver metabolism throughout embryogenesis and at hatch The livers of 8 mule duck embryos were collected every four days from the 12th day of embryogenesis (E12) until after hatching (Hatch/E30) for the daily kinetic and after the increase of temperature (30min, 1h, 2h, 3h, 4h, 5h and 7h), and gene expression analysis was performed. The expression levels of 95 mRNAs were quantified for these 13 sampling points and classified into major cellular pathways. GA_RT-daily and hourly kinetics-mule duck-qPCR EvaGreen Biomark