Effect of E-64 on the motility of adult female S. cervi parasite.

The motility of the parasites was visually checked at different time intervals. Adult female worms (n = 10) of equal size were incubated with 5, 10, 20 and 40 μM concentration of E-64 in 20 ml maintenance medium at 37°C and 5% CO2 for 8 h. Worms incubated in only maintenance medium served as Control. Worm motility was scored as -, no movement; +, least active; ++, less active; +++, moderately active; and ++++, highly active. ©Worms were transferred into fresh medium (devoid of E-64) after 8 h and motility recovery in treated group was compared to control group. Results are from three independent experiments performed in duplicates.