Fig 3 – figure supplement 2: Sleep/wake histories for additional ex vivo slice recordings

Each experiment includes an example hypnogram from one animal above summary data of wake history. (A) Animals from visual cortex L4 recordings. Top figure, example hypnogram from a single animal. Green bars indicate instances of wake, magenta indicates REM, and blue NREM. Below, this same behavioral state data is represented as a moving average of % time spent awake in the preceding 4 hr but for each animal included in the experiment; the dashed lines represent the thresholds for achieving the required density in a given state, and the dots represent endpoints for slice recordings. Black circle around end point dot indicates which trace is represented by example hypnogram. (B) Same as (A) but for wake dense recordings (with green dots). (C-D) Same as (A-B) but for prefrontal cortex L2/3 recordings. (E) Same as (D) but for the D. wake dense recordings (i.e. sacrifice occurred in the dark period).