Bisulfite sequencing PCR combined with HiSeq sequencing (Hiseq-BSP) data of jejunum tissues from Intrauterine Growth Retardation (IUGR) and their normal weight born (NBW) littermates

For validation of some possible important differential methylated region genes (DMRGs), the bisulfite sequencing PCR combined with HiSeq sequencing of these objective genes (MTMR1, HAUS7, FAM127C, AIFM1, PIM2, TWIST2, IRAK1, BCAP31, and SOX3) were performed. The PCR primers of these genes were designed using the online MethPrimer software (www.urogene.org/methprimer/index.html). 400 ng of DNA samples were converted using ZYMO EZ DNA Methylation-Gold Kit™ (ZYMO) and one-third of the elution products were used as templates. PCR amplification was carried out and then PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN) and subcloned. Related colonies from each product were sequenced using the 3730 Genetic Analyzer (Applied Biosystems).