Supporting Data for: Differential gene expression associated with a floral scent polymorphism in the evening primrose Oenothera harringtonii (Onagraceae)

Background: Plant volatiles play an important role in both plant-pollinator and plant-herbivore interactions. Intraspecific polymorphisms in volatile production are ubiquitous, but studies that explore underlying differential gene expression are rare. Oenothera harringtonii populations are polymorphic in floral emission of the monoterpene (R)-(-)-linalool; some plants emit (R)-(-)-linalool (linalool+ plants) while others do not (linalool- plants). However, the genes associated with differential production of this floral volatile in Oenothera are unknown. We used RNA-Seq to broadly characterize differential gene expression involved in (R)-(-)-linalool biosynthesis. To identify genes that may be associated with the polymorphism for this trait, we used RNA-Seq to compare gene expression in six different Oenothera harringtonii tissues from each of three linalool+ and linalool- plants. Results: Three clusters of differentially expressed genes were enriched for terpene synthase activity: t...背景：植物挥发物在植物-传粉者互作及植物-植食者互作中均发挥重要调控作用。挥发物产生的种内多态性现象广泛存在，但针对其背后差异基因表达机制的研究仍较为匮乏。哈氏月见草（Oenothera harringtonii）种群在单萜(R)-(-)-芳樟醇的花部释放性状上存在多态性：部分植株可释放(R)-(-)-芳樟醇（芳樟醇+植株），其余植株则不释放该物质（芳樟醇-植株）。但目前尚不明确哈氏月见草中与该花部挥发物差异产生相关的功能基因。本研究通过RNA测序（RNA-Seq）对(R)-(-)-芳樟醇生物合成相关的差异基因表达进行了广谱表征。为鉴定可能与该性状多态性相关的基因，我们利用RNA测序技术，比较了3株芳樟醇+植株与3株芳樟醇-植株各自6种不同哈氏月见草组织的基因表达水平。 结果：存在3个差异表达基因簇富集了萜合酶（terpene synthase）活性：t...