Histologic, biochemistry, and metabolomics data for bearded dragons with hepatic fat accumulation

Forty-eight adult bearded dragons were used in this study to explore the associations between plasma metabolites, plasma biochemsitry, and plasma liporproteins and hepatic fat accumulation.

Plasma was submitted for untargeted primary metabolomics using gas chromatography time-of-flight mass spectrometry, a biochemistry panel, and a lipoprotein panel determined by high-resolution polyacrylamide gel electrophoresis. Hepatic lipid content was quantified by liver attenuation measurements from computed tomography images and digital image analysis of standardized histologic sections of the liver. Fibrosis was quantified by digital image analysis on Masson’s trichrome stained histologic sections. Severity was determined from pathologic review of liver sections according to a standardized grading system.

Methods
-Histopathology grading

-Digital image analysis using QuPath and ImageJ

-Biochemistry obtained with Roche Cobas c501

-Metabolomics data obtained by gas chromatography mass spectrometry

-Lipoprotein profiles obtained by high resolution polyacrylamide gel electrophoresis (Lipoprint system)