Investigating a novel multiplex proteomics technology for detection of changes in serum protein concentrations that may correlate to tumor burden

We applied the Quantibody® Human Kiloplex Array to measure 1,000 proteins in sera obtained pre- and post-surgically from 5 pancreatic cancer patients. Sera from two healthy individuals, split into two aliquots each, were used as controls. We found nine proteins which decreased significantly post-surgery and may correlate to tumor burden. To validate the multiplexed results, we used single- target ELISA assays to measure the proteins with the largest serum concentration changes after surgery in sera collected pre- and post-surgically from the previous 5 patients and 10 additional patients.
Only 2 of the 8 proteins tested were validated using the ELISA kits.