Poison frog microbiome metagenomics data

Shifts in microbiome community composition can impact function, and in host-associated microbial communities, these functional changes can have ramifications for the host in addition to the microbes. It is of interest to understand how perturbations, like those caused by the introduction of exogenous chemicals, modulate microbiome community composition. In poison frogs within the family Dendrobatidae, the skin microbiome is exposed to alkaloids that the frogs sequester from their diet and use for defense. There are demonstrated antimicrobial effects of these poison frog alkaloids, however how they affect the structure of the skin microbial community is unknown. To test this, we first characterized microbial communities from field-collected Dendrobatid frogs across a range of alkaloid levels. Then we conducted a laboratory experiment to monitor the effect of the common, commercially available alkaloid decahydroquinoline (DHQ) on the microbiome of two frog species with contrasting alkaloi..., , , # Metagenomics data

[https://doi.org/10.5061/dryad.2ngf1vhzc](https://doi.org/10.5061/dryad.2ngf1vhzc)

The metagenomic data come from two species of frogs collected in the wild, *Oophaga sylvatica* and *Allobates femoralis,* as well as samples from these frogs reared in the laboratory, before (Day 12) and after (Day 19) being fed with the alkaloid decahydroquinoline.  

## Description of the data and file structure

See `Metagenomics_metadata.xlsx` for details on specimen. 

* **Sample Number**: Sample identifier, corresponds with FASTQ file names. 
* **Experiment Type**: Either field (samples taken from frogs from the wild) or lab (samples taken from frogs in related alkaloid feeding experiment)
* **Frog Number**: Either a number associated with samples from the wild, or the frog identifier from the feeding experiment. Blanks mean frogs were not assigned a number. 
* **Frog Species**: Either Oophaga sylvatica (Sylvatica) or Allobates femoralis (Femoralis)
* **Treatment**: For those ...