Ct values, final intensities and sequencing results from triplicate nested TaqMan Real-Time PCR reactions for packed cell samples from wild `amakihi with presumptive pox lesions.

Successful amplifications (Ct425) are indicated in bold. All values are based on PCR Base Line Subtracted Curve Fit Data as calculated using iCycler version 3.1 software (BioRad). The threshold intensity was 37.0 rfu for the first reaction, 48.3 rfu for the second reaction, and 10.8 rfu for the third reaction.1A dash (−) indicates a failed first reaction and potential false negative, N/A indicates that no Ct value was assigned because the signal for the sample never reached the threshold intensity for that reaction.2Variant numbers correspond to Avipoxvirus clusters 1 and 2 as previously described [8]; NS indicates not sequenced.