Toxicity of pro-oxidant molecules towards macrophages at different temperature.

Macrophages are either untreated or treated with Methemoglobin, β-hematin or combination of β-hemain/Methemoglobin mixture at 37°C or subpermissive temperature (10°C) for 6 hr and the macrophage survival was measured by MTT assay. The experiment is performed in triplicate and values presented were the mean ± SD of four different experiments (n = 4). The MTT absorbance (0.37±0.05) of untreated cells is considered as 100% and used to express the survival of macrophages in other conditions.