Climate change and urchin fertilisation and the effect on the growth rate of juvenile Abatus sp.

The effect of pH, temperature and sperm concentration on the fertilisation of Sterechinus neumayeri was investigated. Adult Sterechinus neumayeri were collected from Ellis Fjord Narrows between December and January 2011-12 and held in the Ecotox Field Aquarium Module until used. Between 3-4 male and female individuals were spawned using 0.5M KCl and gametes were collected separately before being fertilised in treatment. The data set shows the percentage of fertilised and non-fertilised eggs of Sterechinus neumayeri scored at 20h post-fertilisation. Eggs were fertilised in various combinations of pH, temperature and sperm concentration treatments (pH: 8.0 (Control), 7.8 and 7.6; Temperature: 1 degrees C (Control), 3 degrees C and 5 degrees C; Sperm concentration (sperm:egg ratio): 1000:1 (Control), 750:1, 250: 1, 50:1 and 5:1). At 20h post fertilisation, 5 ml aliquot was removed from fertilisation vials and eggs were counted and determined if they were fertilised or not. Seawater parameters of treatments were measured at the start and end of the experiment.Detailed information of the spreadsheets are as follows:Seawater Parameters column headings:Temperature - measured in degrees C , shows the temperature treatments usedpH - shows the pH levels usedSubheading pH - pH level measured for the day using NIST certified buffersSubheading MV - pH level measured for the day in millivoltsSubheading Total pH - total pH level in seawater obtained from MV measurementsSubheading Temp - temperature of seawater measured for the day1 deg C column headings:Experiment - number of experiments pH - shows the pH for each treatmentSperm Concentration - shows the sperm concentration used for each treatment in a egg:sperm ratioRep - shows the number of replicates per experimentUnfertilised eggs - eggs without visible fertilisation envelope and no cleavage after 20hFertilised eggs - eggs with visible fertilisation envelope and/or cleavage after 20hFertilised deformed eggs - eggs with visible fertilisation envelope but deformedTotal eggs - total eggs scored (whether fertilised or unfertilised)% Fertilised - fertilised eggs (deformed and non-deformed)/Total eggs3 deg C and 5 deg C have the same column headings as 1 deg C.AAS3134 Abatus sp Growth Experiment Davis 2011-12:The effect of pH and temperature on the growth rate of juvenile Abatus ingens and Abatus shackletoni were investigated. Adult Abatus were collected off Airport Beach in waters 4-5m depth. Data set shows the growth rate of juveniles of Abatus ingens and Abatus shackletoni after a 4-week exposure to various combinations of pH and temperature. Juveniles of each species was removed from maternal pouches and photographed on the oral side before being exposed to combinations of pH (8.0 (Control), 7.8 and 7.6) and temperature (-1 degrees C (Control) and 1 degrees C) levels. They were incubated in treatments for 4 weeks before being removed and rephotographed. The lengths of 10 spines per juvenile were measured in the pre- and post-experiment photographs using ImageJ and the difference calculated to get a growth rate per juvenile. Seawater parameters of treatments were measured at the beginning of the experiment and subsequently once a day until the end of the experiment.Detailed information of the spreadsheets are as follows:A ingens (pre-exp) i.e. juvenile Abatus ingens spine lengths measured before exposure to experimental treatments. Column headings are:Spine number and length (mm): Length of each spine (1 - 10) measured per juvenile in mm.R1 - R12: Number of juveniles A ingens (post-exp) i.e. juvenile Abatus ingens spine lengths measured after 4-week exposure to experimental treatments. Column headings are identical to the above.A shackletoni (pre-exp) i.e. juvenile Abatus shackletoni spine lengths measured before exposure to experimental treatments. Column headings are identical to the above.A shackletoni (post-exp) i.e. juvenile Abatus shackletoni spine lengths measured after 4-week exposure to experimental treatments. Column headings are identical to the above.2011-12 Aquarium pH and temp main headings show different treatment parameters. Column sub-headings are:Date - Date of measured seawater parametersSalinity - salinity of seawater measuredPpm - Amount of CO2 gas pumped into water recorded in parts per millionpH - measured pH of seawater using NIST-certified buffersMV - pH of seawater recorded in millivoltsTotal pH - total pH of seawater derived from MVTemp - Temperature of seawater measured in degrees C.

本数据集探究了pH值、温度与精子浓度对新迈尔强棘海胆（Sterechinus neumayeri）受精作用的影响。2011-2012年12月至次年1月，研究人员从埃利斯峡道浅滩（Ellis Fjord Narrows）采集成年新迈尔强棘海胆，暂养于生态毒理野外水族箱模块（Ecotox Field Aquarium Module）直至实验使用。

采用0.5M氯化钾（KCl）诱导3-4只雌雄个体产卵，分别收集配子后于各处理组中完成受精。本数据集记录了受精后20小时统计的新迈尔强棘海胆受精卵与未受精卵占比。实验设置了pH、温度与精子浓度的多组合处理：pH梯度为8.0（对照组）、7.8与7.6；温度梯度为1℃（对照组）、3℃与5℃；精子浓度以精卵比计，分别为1000:1（对照组）、750:1、250:1、50:1与5:1。

受精后20小时，从受精瓶中取5mL等分试样，计数并判定卵的受精状态。实验处理组的海水参数分别于实验起始与结束阶段进行测定。

电子表格详细信息如下：
#### 海水参数工作表表头
- 温度（Temperature）：以℃为单位，记录实验所用温度处理梯度
- pH（pH）：记录实验所用pH水平
- 子表标题pH：当日使用NIST认证缓冲液测定的pH值
- 子表标题MV：当日以毫伏（mV）记录的海水pH值
- 子表标题总pH（Total pH）：通过MV测量值计算得到的海水总pH值
- 子表标题Temp：当日测定的海水温度

#### 1℃实验组工作表表头
- 实验编号（Experiment）：实验序号
- pH（pH）：各处理组的pH水平
- 精子浓度（Sperm Concentration）：各处理组所用的精卵比
- 重复次数（Rep）：每组实验的重复数
- 未受精卵（Unfertilised eggs）：20小时后无可见受精膜且未发生卵裂的卵
- 受精卵（Fertilised eggs）：20小时后具有可见受精膜和/或发生卵裂的卵
- 受精畸形卵（Fertilised deformed eggs）：具有可见受精膜但形态畸形的受精卵
- 总卵数（Total eggs）：统计的总卵数（无论受精与否）
- 受精率（% Fertilised）：（受精卵含畸形卵）/总卵数×100%

3℃与5℃实验组的工作表表头与1℃组一致。

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### AAS3134 阿巴特海胆属（Abatus sp.）生长实验（Davis 2011-12）
本数据集探究了pH值与温度对幼体英根阿巴特海胆（Abatus ingens）与沙克莱顿阿巴特海胆（Abatus shackletoni）生长速率的影响。研究人员于机场海滩（Airport Beach）附近4-5米水深的海域采集成年阿巴特海胆。

本数据集记录了幼体英根阿巴特海胆与沙克莱顿阿巴特海胆经4周不同pH与温度组合处理后的生长速率。将各物种幼体从母体育幼囊中取出，拍摄口面形态后，置于pH（8.0为对照组、7.8与7.6）与温度（-1℃为对照组、1℃）的多组合处理体系中培养4周，随后取出并再次拍摄。

利用ImageJ软件对实验前后的照片进行测量，统计每只幼体10个骨棘的长度，计算差值得到单只幼体的生长速率。实验处理组的海水参数于实验起始阶段测定，之后每日测定一次直至实验结束。

电子表格详细信息如下：
1. **A. ingens（实验前）**：即实验处理前幼体英根阿巴特海胆的骨棘长度数据。表头为：
- 骨棘编号与长度（mm）：每只幼体10根骨棘的长度（1-10号），单位为mm
- R1-R12：幼体编号
2. **A. ingens（实验后）**：即经4周实验处理后幼体英根阿巴特海胆的骨棘长度数据，表头与上述一致。
3. **A. shackletoni（实验前）**：即实验处理前幼体沙克莱顿阿巴特海胆的骨棘长度数据，表头与上述一致。
4. **A. shackletoni（实验后）**：即经4周实验处理后幼体沙克莱顿阿巴特海胆的骨棘长度数据，表头与上述一致。

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### 2011-2012年水族箱pH与温度主工作表
主表头记录了不同的处理参数，子表头如下：
- 日期（Date）：海水参数测定日期
- 盐度（Salinity）：测定的海水盐度
- Ppm：以百万分比（ppm）记录的泵入水体的二氧化碳气量
- pH：使用NIST认证缓冲液测定的海水pH值
- MV：以毫伏记录的海水pH值
- 总pH（Total pH）：通过MV计算得到的海水总pH值
- 温度（Temp）：以℃为单位测定的海水温度