Metaproteomics of environmental MIC samples

Damage caused by microbially influenced corrosion (MIC) impedes the operability of hydraulic metallic structures and has therefore needs to be researched extensively. The objective of our study was to characterize the microbial processes of freshwater environmental samples under sulphate-reducing conditions. Identification of the enzymatic patterns with iron or hydrogen as electron donor, in the presence or absence of an organic carbon source was performed. Shot-gun metaproteomics of the extracted proteins were measured by LC/MS and the following data includes the MS-spectra of the peptides . The MS-data was compared aganist a custom fasta sequence using Proteome dicoverer software 2.5 to identify the protein expression patterns of the bacteria, when incubated with iron or hydrogen as electron donors. The identified proteins were furthermore analysed by Uniprot ID mapping and KEGG database for the elucidation of the relevant microbial pathways that is CO2 fixation pathways, carbon metabolism, sulfur metabolism and energy metabolsism for the possible corrosion mechanisms/key proteins.微生物诱导腐蚀（microbially influenced corrosion, MIC）造成的损害会阻碍水力金属结构的运行性能，因此亟需开展深入研究。本研究的目标是解析硫酸盐还原条件下淡水环境样本中的微生物代谢过程。本研究分别在有机碳源存在与缺失的条件下，以铁或氢作为电子供体，对酶的表达模式进行了鉴定。通过液相色谱-质谱联用（LC/MS）技术对提取的蛋白质进行鸟枪法宏蛋白质组学（shot-gun metaproteomics）检测，本次公开数据集包含所获得的肽段质谱谱图。使用Proteome Discoverer软件2.5版本，将质谱数据与定制化FASTA序列库进行比对，以鉴定以铁或氢为电子供体培养的细菌的蛋白质表达模式。此外，通过UniProt ID映射与京都基因与基因组百科全书（KEGG）数据库，对鉴定得到的蛋白质进行注释分析，以阐明相关微生物代谢途径，包括二氧化碳固定途径、碳代谢、硫代谢以及能量代谢，从而揭示潜在的腐蚀机制与关键蛋白质。