Regulation of T-cell receptor signaling induced gene expression by the zinc finger transcription factor Ikaros.

Ikaros is critical in setting the threshold for T-cell activation and proliferation. Here we used mature CD8+ T cells from mice lacking the zinc finger 4 of Ikaros (Ikaros-Zn4-/-) to study its role in regulating gene expression at early time points after T-cell receptor stimulation. Overall design: Transcriptome profiling was performed using WT and Ikaros-Zn4-/- naïve CD8+ T cells that were stimulated for 0, 30 or 120 minutes.