S2E Figure

S2E Fig. Lentivirally established CMV promoter-driven tdTomato expression does not induce IFN-β in BC-3 cells and does not correlate with IFN-β expression. BC3-CMVp-tdTomato cells were treated with 20 ng/mL TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”). Where indicated, the cells were also treated with 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”). RNA was isolated from cells collected without sorting (“bulk”) or after sorting based on tdTomato expression (tdTomato+ and -). IFN-β mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. mRNA levels are plotted relative to the lytic+casp-i bulk sample. ns = p>0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001. Two-Way ANOVA followed by Tukey’s post hoc multiple comparisons test. n= 3 for all experiments.