Tuneable-Membrane Protein Containing Floating Bilayers: Enhancement of Complexity Resolution through Differential Hydrogen Labelling

We have developed self-assembled floating supported membranes which contain intergral membrane proteins such as the COVID receptor angiotensin converter enzyme 2 (ACE2). This biologically accurate and easy to assemble sample system is self-assembled onto a Oligo-Ethylene-Oxide (OEG) Self-Assembled Monolayer (SAM) coated gold surfaces using a two step self assembly process. The distance of the resulting floating protein-lipid membranes from the bulk interface can be tuned by changing the solution salt conditions. we are at a stage in this work where we are preparing for publication but require some final data sets where the compositional complexity of the floating membranes is abundantly clear. There for here we wish to undertake some final measurements using lipid and protein deutertation to enhance the clarity of the protein distributions across the membranes.