Gene expression data in U87MG cell line transfected with NeuroD2-overexpression and control vector

NeuroD2 is downregulated in glioblastoma (GBM) tumor samples. The study identifies the genes regulated by NeuroD2 in GBM cell lines grown under hypoxia We used human primeview gene expression microarray chips to assess the mRNA expression profile in response to NeuroD2 overexpression in U87MG cells under hypoxic microenvironment. U87MG cells were transfected with control and NeuroD2-overexpression vector and allowed to grow in hypoxic microenvironment (0.2% oxygen in in vivo200 hypoxia workstation from Ruskinn, UK). The total RNA from cells was harvested 48 hours post transfection and mRNA profiling was done using human primeview gene chips.