SAIMOS - Biological and Flow Cytometry data collected from sampling stations in South Australia, in August 2019

Flow cytometry data was collected in August 2019, in waters off South Australia, from the Gulf St Vincent (SAMGSV). The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.Since July 2019 two additional BGC stations have been added to the SAIMOS sampling array: the SA Upper Spencer Gulf Mooring (SAMUSG, 33.111S, 137.709E) and the SA Gulf St Vincent Mooring (SAMGSV, 35.023S, 138.46E).

2019年8月，研究团队于澳大利亚南部海域圣文森特湾（Gulf St Vincent，SAMGSV）采集了流式细胞术（Flow Cytometry）数据。
本研究的核心目标是为澳大利亚南部大陆架海域的生态系统建立背景知识库，阐明上升流与盐度外泄事件对微生物群落的影响，并最终构建该区域的生物地球化学模型。
本次采样依托“恩格林号”科考船（RV Ngerin）开展，隶属于南澳大利亚综合海洋系统（Southern Australian Integrated Marine System，SAIMOS）。
每趟航次中，研究人员均对叶绿素荧光最大值层（Deep Chlorophyll Maximum，DCM）的物理、化学及生物学特性展开系统性调查。
本数据集涵盖了针对微微型浮游植物、细菌与病毒的流式细胞术检测数据。
本研究共设置6个核心采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）与SAM5CB/B5（35.008°S，135.198°E）；剩余1个为外海站位B1（36.188°S，136.178°E），地处袋鼠岛西南海域。
需特别说明的是，结合站位间距（14~25海里）、陆架中层海流的平均流速分量（0.01 m·s⁻¹）以及科考船的平均航速（约9节），可明确各站位所采集的为不同水团样本。
研究团队还偶尔在袋鼠岛国家参考站（National Reference Station，NRS，35.832°S，136.447°E）以及发生盐度外泄事件的南澳斯宾塞湾口锚站（SAM8SG，35.25°S，136.690°E）采集补充样本。
自2019年7月起，SAIMOS采样阵列新增2个生物地球化学（Biogeochemical，BGC）站位：南澳斯宾塞湾上游锚站（SAMUSG，33.111°S，137.709°E）与南澳圣文森特湾锚站（SAMGSV，35.023°S，138.46°E）。