Transcript abundance comparison between uninfected DCs and DCs housing L. amazonensis

To determine the modulation of gene expression of mouse BMDCs in the presence of living intracellular Leishmania amazonensis amastigotes Dendritic cells were generated in vitro from bone marrow progenitors. Leishmania amazonensis amastigotes were purified from mouse cutaneous lesions and were added to DC cultures. After 24h, and following a sorting procedure, only BMDCs housing living amastigotes were selected for total RNA extraction based on the expression of MHC class II (host cell) and DsRed2 (parasites) molecules.