Miguel Vicente-Manzanares, Karen Newell-Litwa, Alexia I. Bachir, Leanna A. Whitmore, Alan Rick Horwitz (2011) CIL:25843, Cricetulus griseus, epithelial cell. CIL. Dataset

Time-lapse microscopy of a spreading control Cho-K1 cell expressing RLC-D,D–GFP (a phospho-mimetic mutant of the RLC) on fibronectin. The arrow points to the site of initial accumulation of RLC-D,D–GFP. Note the protrusion opposite the concentration of RLC-D,D, which results in cell polarization and definition of the rear. Images were captured in TIRF mode using an inverted microscope (IX70; Olympus) coupled to a CCD camera (Retiga Exi; Qimaging). Live samples were acquired at 37°C using CCM1 medium. This movie corresponds to Fig. 1 C. and Video 1 from J Cell Biol 2011 193:381-396.

本数据集记录了表达RLC-D,D–GFP（一种RLC的磷酸模拟突变体）的Cho-K1细胞在纤连蛋白上的扩散过程的时间序列显微照片。箭头所指为RLC-D,D–GFP初始聚集的位点。请注意，RLC-D,D的浓度相反端形成的突起，这导致了细胞的极化和后端的界定。图像采用倒置显微镜（IX70；Olympus）结合CCD相机（Retiga Exi；Qimaging）在TIRF模式下捕捉。活体样本在37°C下使用CCM1培养基获取。本视频与J Cell Biol 2011年第193卷第381-396页中的图1C和视频1相对应。