A study of A bacterial host factor confines phage localization for excluding the infected compartment through cell division. Pollak Fiyaksel et al.

This dataset supports the research described in our manuscript: "A bacterial host factor confines phage localization for excluding the infected compartment through cell division." Our hypothesis was that Bacillus subtilis actively restricts bacteriophage infection through a host-driven mechanism. The data demonstrates that the bacterial protein YjbH binds to the injected phage DNA and recruits the cell division machinery to isolate the infected region via asymmetric division, ultimately confining the spread of viral components. The dataset includes: * Raw and processed images from fluorescence and confocal microscopy, tracking phage infection dynamics. * Quantifications of plaque size, lysis curves, and protein–DNA binding assays (e.g., EMSA, ddPCR). * Western blot data for protein expression validation. * Cryo-ET tomograms showing asymmetric septa formation. * DNA-protein interaction data, including co-IP and MS results. Each dataset is labeled by figure and panel, and includes a short description to facilitate interpretation.本数据集为本课题组发表于题为《细菌宿主因子通过细胞分裂隔离感染区室以限制噬菌体定位》的手稿中的相关研究提供数据支撑。 我们提出的假说为：枯草芽孢杆菌（Bacillus subtilis）可通过宿主介导的机制主动抑制噬菌体感染。研究数据显示，细菌蛋白YjbH可结合注入宿主的噬菌体DNA，并招募细胞分裂装置，通过不对称分裂隔离感染区域，最终限制病毒组分的扩散。 本数据集包含以下内容： * 用于追踪噬菌体感染动态的荧光显微镜（fluorescence microscopy）与共聚焦显微镜（confocal microscopy）原始及处理后成像数据； * 噬菌斑尺寸、裂解曲线以及蛋白质-DNA结合实验（如电泳迁移率变动分析（electrophoretic mobility shift assay，EMSA）、数字液滴PCR（digital droplet PCR，ddPCR））的定量数据； * 用于验证蛋白表达的蛋白质印迹（Western blot）实验数据； * 显示不对称隔膜形成的冷冻电子断层扫描（cryo-electron tomography，Cryo-ET）断层图数据； * 包含免疫共沉淀（co-immunoprecipitation，co-IP）与质谱（mass spectrometry，MS）结果在内的DNA-蛋白质相互作用数据。 每份数据集均按图片及子图进行标注，并附带简短说明以辅助解读。