Clonal diversity of peripheral B cell receptor repertoire for prediction of relapse risk after autologous stem cell transplant in DLBCL patients

The goal of this study is to analysis the gene expression alterations along the time of hiGC culture. We established a hiGC model by following the protocol previously reported by Kitamura et al. Naïve B cells purified from human PBMC pools underwent two phases of growth under the activation of CD40L, BAFF, IL-4 and subsequently IL-21. Overall design: There are 5 samples on different culturing time.