Genome-wide Analysis of mRNAs, lncRNAs, and circRNAs during Intramuscular adipogenesis in Chinese Guizhou congjiang Pigs Breeds

Intramuscular fat content plays an important positive role in meat quality. Preadipocyte differentiation plays a critical role in intramuscular fat deposition in pigs. However, many types of RNAs, including messenger RNAs (mRNA), long noncoding RNAs (lncRNA) and circular RNAs (circRNAs), play crucial roles in regulating fat in adipogenesis and differentiation are still unreported. Chinese Guizhou congjiang pigs are an important pig breeds raised for meat in the Guizhou province of China because of its good meat quality. Therefore, it is very important for breeders to explore the mechanism of proliferation and differentiation in intramuscular adipocytes from the longissimus dorsi muscle of Chinese congjiang pigs. In present study, a transcriptome analysis, including the analysis of mRNA, lncRNA and circRNA, during different differentiation stages, namely, day 0 (D0), day 4 (D4), day 8 (D8), of intramuscular preadipocytes from Chinese Guizhou congjiang pigs was performed. A total of 1538, 639, and 445 differentially expressed (DE) mRNAs, 479, 192, and 126 DE lncRNAs, and 360, 439, and 304 DE circRNAs were detected between D4 and D0, between D8 and D0, and between D8 and D4, respectively. Functional analysis showed many significantly enriched lipid deposition, cell differentiation, metabolism process, and obesity-related diseases biological processes and pathways. We identified two lncRNAs, TCONS_00012086 and TCONS_00007245, which are closely related to fat deposition according to their target genes and detected their tissue expression profile. In conclusion, these data provide a genome-wide resource of mRNAs, lncRNAs and circRNAs potentially involved in Chinese Guizhou congjiang pig fat metabolism, thus improving understanding of their function in diverse adipose.