Effect of HU treatment on gene targeting efficiency.

Transformants of Y. lipolytica, A. adeninivorans, S. cerevisiae, P. pastoris and K. lactis untreated or pretreated with HU were screened to distinguish random and targeted integration events. The percentage of gene targeting is shown and the number of total transformants screened is included in parentheses. Targeted genes are listed by their systematic names (Y. lipolytica, S. cerevisiae, P. pastoris K. lactis) or GenBank accession numbers (A. adeninivorans). The Fisher’s exact test hypergeometric probability for each individual experiment is tabulated in the last column.