Data files Prmt7 and adipocyte differentiation

Dataset 1: Dataset for Fig.1B<br>Quantification of Prmt7 protein levels in differentiating C3H10T1/2 cells. Column A describes the time when cells were harvested. Columns B to E show the signal intensities of Prmt7 and PI3K from two independent experiments, quantified by ImageJ. Columns F and G show the ratio of Prmt7 levels to PI3K levels for each sample in each experiment. Columns H and I show relative Prmt7 levels where the day 0 sample in each experiment is normalized to 1. Column J shows the mean of the relative Prmt7 levels from the two independent experiments. Dataset 2: Dataset for Fig.2B<br>Quantification of Prmt7 protein levels in the knockdown and over-expression C3H10T1/2 cells. Column A shows the names of the cells. Columns B to E show the signal intensities of Prmt7 and PI3K from two independent experiments, quantified by ImageJ. Columns F and G show the ratio of Prmt7 levels to PI3K levels for each sample in each experiment. Columns H and I show relative Prmt7 levels where the shControl and the pBABE vector sample in each experiment is normalized to 1. Column J shows the mean of the relative Prmt7 levels from the two independent experiments. Dataset 3: Dataset for Fig.3<br>Cell counting results. Column A shows the time when cell numbers were counted. Column B shows the names of the cells. Columns C to F show the numbers of the cells (x105) from two independent experiments with two replicates in each experiment. Column G shows the mean of the cell numbers. Column H shows the standard deviation (SD) of the cell numbers. Dataset 4: Dataset for Fig.4B<br>Quantification of Oil-Red O staining on day 6 post-differentiated C3H10T1/2 cells. Column A shows the names of the cells. Columns B to Column E show the values of OD500 from two independent experiments with two replicates in each experiment. Column F represents the mean of the OD500 values. Column F shows the mean values. Column G shows the standard deviation (SD) of the OD500 values. Dataset 5: Dataset for Fig.4D<br>Real-time qPCR results for the expression of selected genes in day 6 post-differentiated C3H10T1/2 cells. Column A shows to the names of the transcripts. Column B shows the names of the cells. Column C indicates the experiment number. Columns D and E show raw Ct values from two replicates from each sample. Column F shows the mean raw Ct value. Columns G to K show the following values: delta Ct (Ct – Ct5S rRNA), delta delta Ct (dCt - dCtshCtrl), fold change (2^(-ddCt)), the mean of the fold change, and the standard deviation (SD) of the fold change.