Fusarium protease reduction of gluten strength

Development of fluorescence assay for Fusarium protease damage to gluten, Extensigraph Rmax characterization of blends of Fusarium damaged seed into sound base, Fusarium protease analysis of single infected seeds and digestion of bovine beta casein using sound and Fusarium damaged seed extracts as enzyme source. Data shows increase in protease with infected seed content and reduction in Extensigraph Rmax (indicator of gluten strength). Digestion of beta-casein indicates preference for K at P1 position, isoleucine at P1 and P1', arginine at P1' and tyrosine at P1.本研究开发了针对镰刀菌（Fusarium）蛋白酶破坏谷蛋白（gluten）的荧光检测方法；开展了以健康基底原料与镰刀菌侵染种子的混合物为测试样本的面团拉伸仪（Extensigraph）Rmax值表征实验；完成了单粒侵染种子的镰刀菌蛋白酶分析，以及以健康种子和镰刀菌侵染种子提取物作为酶源，对牛β-酪蛋白（beta casein）进行酶解的相关实验。实验数据表明，蛋白酶活性随侵染种子占比升高而上升，同时面团拉伸仪Rmax值（谷蛋白强度的表征指标）呈下降趋势。β-酪蛋白的酶解结果显示，该蛋白酶对酶切位点存在如下偏好性：P1位偏好赖氨酸（K）、P1与P1'位偏好异亮氨酸、P1'位偏好精氨酸，且P1位偏好酪氨酸。