Identification of Nucleolin-binding targets and sequences in LPS-stimulated RAW 264.7 cells by PAR-CLIP

To investigate the consensus RNA-binding motifs of Nucleoli, we performed photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) analysis in nucleolar fractions from unstimulated or 12 h LPS-stimulated RAW 264.7 cells We obtained NCL-bound RNAs from the nucleolar fractions of 12 h LPS-stimulated RAW264.7 cells and analyzed PAR-CLIP data using PARpipe analysis.